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Gram Negative Unknown Lab Report Using comparative analysis several biochemical tests were performed to determine which bacterium out of the six potential unknowns was given. In order to determine the microorganism characteristics the sample was report for unknown isolated using a t-streak and see more colonies were gram stained to reveal sample microbiology lab report for unknown shape and morphology sample microbiology lab report for unknown then inoculated into several harvard admission college-admission-essays.com of media corresponding with the proper biochemical test.

After allowing the corresponding time for each biochemical test, data was collected to determine the unknown bacteria. The broth culture in this experiment was determined as Escherichia coli.

Introduction All organisms are divided into three domains; bacteria, archaea, and eukarya. The organisms making up domain Bacteria and domain Archaea are all prokaryotes. Although bacteria and archaea look the same, report for unknown is more closely related to eukarya Madigan et.

The ability report for unknown adapt to a broad range of habitats helps to explain why prokaryotes report for unknown the most abundant organism on earth. The main characteristics of a prokaryote include, sample microbiology lab nucleus, circular DNA, and no membrane bound organelles.

Most bacterial cell report for unknown contain peptidoglycan, a network of modified-sugar polymers cross-linked by short polypeptides. All known pathogenic bacteria fall under prokaryotes, but not all bacteria are pathogenic Madigan et. Using a differential staining technique bacteria can be divided into two groups; gram positive or gram negative.

The gram-positive bacteria have thick cell wall made of peptidoglycan. Gram-negative bacteria have report for unknown layer of peptidoglycan, and are structurally more complex, with an outer membrane that contains lipopolysaccharides.

Gram staining is a valuable tool to determine whether an unknown bacterium is gram negative or gram positive. Samples are first stained with crystal dye and iodine, then rinse in alcohol, and finally counterstain with sample microbiology lab report for unknown. In gram-positive bacteria the crystal violet adheres to the peptidoglycan layer staining lab report purple.

In gram-negative the crystal violet is easily removed due /how-to-write-a-creative-paper-critique.html the thinner layer of peptidoglycan making the cell appear pink or red.

The domain bacteria is very diverse, making it impossible to identify it unknown the use of staining and biochemical tests. Biochemical tests can test for a variety of characteristics such as shape, morphology, pH, etc. Tests such as Methyl Red and Voges- Proskauer check for glucose fermentation. The Citrate Test is a nutrient utilization test that determines whether a bacterium produces lab report for enzyme citrate- permease sample microbiology be utilized as its sole source of carbon.

Urease test unknown used sample microbiology indicate development phd thesis a bacterium can hydrolyze the intracellular enzyme urea.

Gram Negative Unknown Lab Report # 31 | Diana Monroe -

SIM gives three test results; Sulfur reduction, which determines whether a bacterium can use report for unknown to reduce sulfur to hydrogen sulfide, Indole production used to determine if the bacteria can hydrolyze tryptophan sample microbiology lab report for unknown pyruvate, ammonia, and motility when in the sample microbiology lab report for unknown media a motile bacteria can diverge from the stab line. TSIA is designed to differentiate bacteria with reference for unknown glucose fermentation, lactose fermentation, sucrose fermentation, and sulfur reduction Leboffe Materials and Method To prevent errors in any procedure personal protective and equipment aseptic technique was strictly used.

These techniques included sterilizing the loop on the Bunsen burner between inoculations and flaming the opening of the test tube before inserting in the loop with the bacteria.

The plate was incubated for 24 hours in a in a 37 degree Celsius room and then transferred to a cold sample microbiology lab 4degree Celsius room.

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