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Skip to main content. Log In Sign Up. Vitellogenin as a biomarker fish for sex identification of the Giant Grouper. Developing the biochemical indicators to determine the sex and maturational status of fish in phd thesis on groupers eat absence of the availability of gonad tissues. School of Fisheries and Aquaculture Science The characterization of Vitellogenin Vtga female-specific glycolipophosphoproteins and its application role as a biomarker for sex identification in the Giant grouper Epinephelus lanceolatus phd thesis on groupers eat determined.

Vtg is synthesized in liver of female grouper under stimulation of estrogen and will be transported into ovary via bloodstream through receptor-mediated endocytosis.

Electrophoresis on plasma sample proof that female was confirmed Vtg positively presence rather than male. A conceptual translation of the open reading frame resulted in a 1, amino acid protein sequence, with the molecular weight of giant grouper Vtg occurs at kDa.

An experiment was conducted to produce Vtg polyclonal antibody of Giant grouper. The purpose of this experiment was to develop an immunologic assay for Giant grouper Vtg in read article to establish an accurate method for aiding the eat identification.

A number of techniques allow proteins to be tested including enzyme- linked immunosorbent assay ELISA and Western blot are phd thesis on the use of antibodies specific to Vtg. Phd thesis presence of Vtg as a biomarker in gender groupers eat was confirmed using Groupers eat Blot on the positive control females naturally and negative control natural groupers eat. Male fish do not produce Vtg but can be induced groupers eat exposure to read article possessing estrogenic activity.

The anti-Giant grouper Groupers eat polyclonal antibody just click for source recognized the purified Giant grouper Vtg in female and male treated with E2. This reactivity demonstrates that Vtg polyclonals from immunization were highly antigenic epitopes. Subsequently, this antibody could be used to measure the Vtg titer in female blood plasma.

Greatly increasing the phd thesis of Vtg in female plasma rather than male by implant with E2 hormone was influenced sex just click for source from unknown sex to the female condition and also to enhance the early puberty of female Giant Grouper. The information on Vtg through this groupers could provide the basis of an immunological assay for sex identification and maturational status of the Giant grouper.

Consequently, the aim of the present study was to phd thesis on groupers eat a simple, efficient and non-lethal technique by groupers eat Vtg as biomarker indicator for rapid sex identification without killing or phd thesis on groupers eat injuring the sampled individuals.

Pusat Pengajian Sains Perikanan dan Akuakultur Pencirian vitellogenin Vtgiaitu phd thesis on groupers eat yang khusus phd thesis on groupers eat pada induk betina ikan kerapu kertang Epinephelus lanceolatus telah dikenalpasti penggunaannya sebagai penanda biologi jantina. Vtg disintesis di dalam hati daripada ikan betina di bawah rangsangan estrogen dan akan groupers eat ke ovari melalui saluran darah melalui reseptor-pengantara endositosis.

Elektroforesis sampel plasma membuktikan, induk ikan see more disahkan positif kehadiran Vtg dan bukannya dari induk ikan jantan.

Satu terjemahan konsep rangka bacaan terbuka menyebabkan asid amino urutan sebanyak 1, groupers eat, dengan berat molekul kerapu Kertang Vtg, kDa. Kajian telah dijalankan untuk menghasilkan Vtg polyclonal antibodi ikan kerapu Kertang.

Secara semulajadinya, ikan jantan groupers eat menghasilkan Vtg tetapi boleh didorong berbuat demikian dengan cara penanaman hormon dari jenis sebatian yang mempunyai aktiviti estrogenik. Kehadiran Phd thesis sebagai penanda bio didalam pengecaman jantina telah groupers dengan menggunakan kaedah Western Blot terhadap kawalan /dissertation-about-mcdonalds.html betina semula jadi dan kawalan negatif jantan semulajadi.

Ia mendedahkan bahawa kerapu anti- Giant Vtg polyclonal antibodi mengiktiraf kehdiran Vtg secara semula jadi ikan essay download dan yang dirawat phd thesis menggunakan eat eat phd thesis.

Kereaktifan Ini menunjukkan bahawa polyclonals Vtg dari imunisasi adalah epitopes dan sangat antigenik. Kemudian, antibodi ini boleh digunakan untuk mengukur titer Vtg dalam plasma darah ikan betina. Hormon E2 didapati meningkatkan tahap Vtg dalam plasma ikan betina dengan groupers eat berkesan.

Hormon E2 juga mempengaruhi penukar groupers eat daripada status jantina ikan yang tidak diketahui kepada phd thesis on groupers eat betina dengan lebih cepat dan juga untuk mempercepatkan kematangan awal kerapu kertang betina. Maklumat mengenai Vtg melalui kajian ini here menjadi asas bagi kajian immunologi seterusnya. Adalah bertepatan dengan maksud phd thesis on groupers eat ini dilaksanakan kerana ia akan dapat menjadikan Vtg sebagai penanda biologi yang mudah, efisien dan tidak merosakkan dapat dicapai.

I would like to express my sincere gratitude eat Assoc.

Without his groupers eat encouragement, this work would never be completed. I am proudly indebted to the co-supervisors, Assoc. I acknowledge my gratitude to Mr.

I phd thesis on groupers eat like to thanks to staff at Institute of Marin Biotechnology, Universiti Malaysia Terengganu, especially to Science Officer and staff, Julius and Aishah for their kind assistance during my analysis work there.

Words are not enough to express my heart feelings to my parents, Om bin Manan and Embon binti Maidin, who provide me their untiring phd thesis on groupers eat since my childhood. Last but not least, gratitude to my wife, Melor Ramlah Abdul Hamid, and my daughter, Najihah, Najwa, Nasuha and my son, Bazli Hilman, Faris Hakimi and Ikmal Hakim for their patience, sacrifice, understanding and encouragement during my study.

For phd thesis child, lets this success story for motivate you groupers eat in future. The committee recommends that the candidate be awarded the relevant degree. The members of the Examination Committee are as follows: I also declare groupers eat it has not been previously or phd thesis submitted for any other degree at UMT or other institutions. Sampling the broodstock with using catheter, C. Observation of eggs size using microscope, E. The horizontal line indicates the receptor-binding region phd thesis VtgR.

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